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Selective alanine transporter utilization creates a targetable metabolic niche in pancreatic cancer

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(Aspirin was used to prevent repeat heart attacks for decades before the F.D.A

The cellular assays, including reactive oxygen species (ROS) assay (4 h-treatment), thiobarbituric acid reactive substances (TBARS) assay (6 h-treatment), glutathione (GSH) assay (6 h-treatment), and cell viability assay (12 h-treatment), were done as previously described [28] using a fluorescence spectrometer (VersaFluor, Bio-Rad Laboratories)

Some studies have lasted over one to two years