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Similarly, scRNA-seq analyses of 5XFAD mice also identified disease-associated astrocyte (DAA) clusters related to AD and aging conditions, in which genes including cstb , serpina3n , vim , gata1 , gsm , and osmr were highly expressed 22

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PubMed,

High titer lentiviral preparation 293T cells were split into 15 cm plates, precoated with 20 g ml 1 of Poly-L-lysin (Sigma-Aldrich, USA), to reach 60% confluency

Jongsma, H

Glia 56, 975989