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For instance, thyroid cancer is associated with the increased presence of Clostridiaceae , Neisseria , and Streptococcus , whereas in patients with thyroid nodules, a relative increase of Streptococcus and Neisseria compared to healthy controls was observed (235)

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RNA extraction, cDNA synthesis and real-time quantitative PCR (RT-qPCR) Total RNA was isolated from cells using a TRI Reagent kit (Sigma) according to the manufacturers instructions

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