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RT-PCR reactions were conducted using a Real-Time PCR system (DTlite, DNA Technology, LLC, Moscow, Russia, 117587) and SYBR Green PCR Master Mix in a 25 L reaction volume

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doi: 10.1016/S0015-0282(00)00518-5 21 ShiMSirardM-A

While this may sound impressive, none of these assertions are based on human trials