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Lets start with the big question everyone asks

Ferris DM, Moodie GD, Dimond PM, Gioranni CW, Ehrlich MG, Valentini RF

reported in an early study that Fas signaling stimulation did not lead to caspases recruitment in erythrocytes [182], it was later demonstrated that phosphatidylserine translocation to the outer membrane in RBCs was regulated by the assembly of the Fas/FADD/caspase-8 complex and downstream activation of caspase-3 [191]

Secondly, the NVT (T = 298 K) and NPT (P = 1.0 bar) ensemble equilibration were performed to stabilize the systems through the velocity rescaling thermostat and Berendsen barostat, respectively

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