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You should always calculate the amount of bac water you need based on your desired final concentration before you start the mixing process

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The mice were anesthetized with 300 mg/kg chloral hydrate and were intratracheally injected with 3 mg/kg BLM (Meilun Biotechnology Co., Ltd., Dalian, China) in 100 l of saline via tracheostomy to induce pulmonary fibrosis