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Western blot results revealed a lower expression of CD31 and VEGF- in the ischemic tissues of GSDMD -/- mice compared to controls (Fig

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E3 ligases in metabolic diseases Insulin receptors and insulin receptor substrates (IRS-1 and IRS-2) are crucial for insulin signaling, and E3 ligases such as MG53, c-Cbl, and Nedd4 can directly degrade these substrates, disrupting signaling and promoting insulin resistance

Anderson, Methods in Enzymo l., 1985 , 113 , 548