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DEGseq method was used to screen for differentially expressed genes 80

Methylated CpG islands are typically associated with heterochromatin, partly due to the recruitment of methyl-CpG binding proteins, which in turn recruit histone deacetylases and other chromatin-remodeling complexes to promote transcriptional repression ( In addition, histone methylation represents another major epigenetic mechanism that regulates chromatin structure and gene expression