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12.5 l of Dream Taq Green PCR Master Mix 2X (Thermo Scientific K1081), 1 l of primer 10 pmol (Metabion, Germany), and 1 l of template DNA (50 ng/L) made up the PCR reaction mixtures

For B12, prolonged use of metformin and acid-suppressing drugs (PPIs, H2 blockers) may reduce absorption of oral B12, which is one reason injectable B12 is considered in select patients

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In line with the in vivo imaging results, the ex vivo imaging of the excised tissues from Cy5.5@vesicles at 6 h p.i

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doi: 10.1186/s13018-023-03673-0 70 HeLZhangYCaoQShanHZongJFengLet al