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Ten microliters of 0.1 mg mL 1 ShHTL7 protein in a reaction buffer containing 20 mM HEPES, pH 7.0, 150 mM NaCl and 80 L of reaction buffer were mixed in a 96-well black plate (Grenier)

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To see this, suppose that the survivors at the last time point in a were heat-tolerant mutants or persistor-like cells

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