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The absorbance at 540 nm was measured with a Wallac Envision 2104 Multilabel Reader (PerkinElmer)

These effects could be attributed to treatment with liraglutide and not to weight loss, since they were not observed in the group of animals that lost weight without receiving the GLP-1RA [40]

But when we did use this, it was pretty benign

Sealed vials are credited with preventing water ingress, while damaged or opened seals are described as compromising both sterility and stability

Target cells were infected and selected with antibiotics (72 h) before functional assays