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If autologous source is used, it requires taking a biopsy quickly, isolating mitochondria (~30 minutes process), and administering them, which may fit in some surgical settings (like before removing cross-clamp in heart surgery)

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Proc Natl Acad Sci U S A (1995) 92(20):9097101.10.1073/pnas.92.20.9097 101 HouJZSchindlerUHenzelWJHoTCBrasseurMMcknightSL

Mahmoodzadeh Y, Mahmoudi J, Gorgani-Firuzjaee S, Mohtavinejad N, Namvaran A

After attaining whole-cell voltage clamp, cells were raised off the dish and positioned near the interface of the theta pipette solution streams