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Naviaux et al., 2019
Tumors were harvested 12 days after initial tumor challenge and dissociated in dissociation buffer rotating at 37C for 30 minutes, passed through a 70 M filter, treated with ACK lysis buffer (ThermoFisher, #A1049201), quenched with RPMI and 10% FBS, and washed in sterile PBS
Nat Rev Drug Discov 2010;9:72843
MOTS-c activates AMPK more acutely through folate cycle disruption and AICAR accumulation
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