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Quantitative real-time polymerase chain reaction (qRTPCR) To determine mRNA expression, total RNA was extracted from each sample group using TRIzol reagent, and cDNA synthesis was performed using a Transcription First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland)

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Added Paradoxine

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The FDA's boxed warnings highlight gastrointestinal side effects like nausea, vomiting, and diarrhea, which usually diminish over time

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