Integration of knockout plasmids was verified by Southern analysis of chromosomes separated by CHEF or FIGE
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The nanomaterials are typically between 0.1 and 100 nm in size, and they exhibit extraordinary capabilities due to higher surfaces-to-volume ratio [7], and they find applications in food science, agriculture, marine science, environmental chemistry, veterinary medicine, medicine detection, and in several other industries [8,9,10,11,12,13,14,15,16]
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