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Thrombospondin from activated platelets promotes sickle erythrocyte adherence to human microvascular endothelium under physiologic flow: a potential role for platelet activation in sickle cell vaso-occlusion

To calibrate the probe, fully oxidized and fully reduced samples were generated by the addition of 20 mM N , N , N , N -Tetramethylazodicarboxamide (diamide, Sigma Aldrich ) or 100 mM Dithiotreitol (DTT, AppliChem ), respectively

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The binding sites of the targets were identified based on their co-crystallized ligands