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The formation of glutathione begins with the conjugation of cysteine and glutamate, then glutathione synthase links this molecule with glycine

Holmes K, Roberts OL, Thomas AM, Cross MJ (2007) Vascular endothelial growth factor receptor-2: structure, function, intracellular signalling and therapeutic inhibition

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Twenty microliters of dilution buffer were added to the Blank 2 and Blank 3 solutions