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This is where the answer gets more nuanced

The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2

Experiments were repeated three times independently, with similar results ( a-h )

Additionally, oxidative stress impairs protein and DNA function, contributing to neuronal dysfunction and death

Mallidis, C

AS160, the Akt substrate regulating GLUT4 translocation, has a functional Rab GTPase-activating protein domain