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This diagram serves as an effective visual tool demonstrating the energy transitions and relaxation processes that occur in molecules after the absorption of light and showing corresponding processes, such as absorption, fluorescence and delayed fluorescence (from singlet states), phosphorescence (from triplet states after intersystem crossing), and non-radiative transitions

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Model building A starting model was assembled using published structures (Protein Data Bank (PDB) IDs: 7K00 (ref

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Peptide Class: Extended single-chain polypeptide Disulfide Bonds: Three intramolecular CysCys bridges Key Modification: Arg substitution + 13-residue N-terminal extension Molecular Formula: CHNOS Appearance: White to off-white lyophilized powder Physicochemical Properties Solubility: Soluble in dilute acetic acid (0.1 M) and aqueous buffers at mildly acidic pH Isoelectric Point: ~8.4 (basic character from Arg/Lys content) UV Absorption: Aromatic residue signal from Tyr and Phe chromophores at 275280 nm Stability: Lyophilized form stable at -20C Analytical Methods: HPLC (purity), mass spectrometry (identity confirmation) FOR RESEARCH USE ONLY

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