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The samples were resuspended in HENS buffer (0.25 M HEPES, 1 mM EDTA, 0.1 M neocuprione, 1% SDS) with 20 mM MMT and incubated for 30 minutes at 50C to block free sulfhydryls

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In accordance with the WHO criteria, the European Food Safety Authority (EFSA) has set the average requirement (AR) of iodine at 150 g/day for adults, 70130 g/day for infants aged 711 months and children, and 200 g/day for pregnant women

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Further studies are needed in this regard