A well-designed glutathione conjugation strategy considers substrate electrophilicity, the role of the cysteine thiol versus terminal amino or carboxyl groups, protection and deprotection needs, solvent and pH compatibility, oxidation risk, and the downstream analytical question the customer actually needs to answer
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Supplementation with folic acid
Sections were collected in 0.1 M phosphate-buffered saline (PBS) and blocked for 1.5 h at room temperature in a blocking solution containing 0.3% Triton X-100, 2% donkey serum, and the appropriate concentration of goat serum in 0.1 M PBS
Lancet Diabetes Endocrinol 2025