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Fermentor runs were initiated by the addition of a 20 ml starter culture grown at 30C to stationary phase in HC medium lacking histidine for plasmid retention

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SELENOP appears to be especially critical for selenium homeostasis in the brain and testes where apolipoprotein E receptor 2 (apoER2) facilitates the uptake of SELENOP

You might get away with it for a single-use application performed in a completely sterile environment, but that rarely describes the reality of most research protocols