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After pre-conditioning, cells were washed and incubated in glucose-free DMEM medium containing 20 M 2-NBDG for 30 min at 37 C

In addition, we found that TG levels in HepG2 cells were not reduced by NaB intervention alone but were markedly reduced by the combination of NaB with GLP-1, suggesting that NaB alleviated hepatic steatosis partially through enhancing GLP-1 action