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25 l was transferred into the Nucleofector cassette and electroporated using the CA-189 protocol (Zou et al, 2021)

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A peristaltic pump was used to continuously pump culture from the fermentor through a flow cell (Type 71-F, Starna GmbH, Pfungstadt, Germany), which was inserted into a JASCO FP-6500 spectrofluorimeter (JASCO, Oklahoma City, OK, United States) before media returned to the fermentor vessel

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