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The assay was performed by heating samples in a Mx3005p qPCR machine (Agilent Technologies, USA) from 25 to 97 C in 1 C min 1 increments for 30 s in an expanding saw-tooth profile, a refinement of a previously described protocol 24

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Decreases in Glutathione is shown to be correlated to all age-related diseases

Additionally, maternal zinc deficiency suppresses the secretion of IFN- from splenocytes and reduces IFN- expression in both CD4 + and CD8 + T cells [384]